primerClassic tool
Annealing Temperature Calculator
Estimate primer Tm and a starting annealing temperature from the sequence.
Use this tool when you need a quick annealing temperature estimate for a short primer without opening a spreadsheet or external software. It counts A, T, G and C bases and applies a simple Tm formula to suggest a starting point.
The result is an initial bench reference. For a final protocol, adjust based on the primer, buffer, instrument and experimental validation.
Use clear inputs to get a more useful result.